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Exfection™ Plasmid EF

Endotoxins (lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. GeneAll® Exfection™ Plasmid EF provides simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.

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Overview

Endotoxins (lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli

It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. 

GeneAll® Exfection™ Plasmid EF provides simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.

01

Format

Exfection™ Plasmid EF utilizes glass microfiber membrane.

  • Spin column format
  • Cell debris and salt precipitates are removed by centrifugation for mini kit and by EzClear™ Filter column for Midi kit
  • Midi kits require the centrifuge which has swing bucket rotor and ability of 4,000 x g at least.
02

High purity

  • Stable and consistent result
  • High yield and purity
  • Endotoxin level : <0.1 EU/μg
03

Simple and safe procedure

  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Suitable for Cell transfection of most cell lines, enzymatic modifications, library construction, in vitro transcription/translation, high quality sequencing, cloning and most molecular biological experiments.

specifications

Technical Information

Features Plasmid EF Midi
Format Column Type E with collection tube
Recommended sample volume30~150 ml LB
Lysate clearingEzClear™ Filter
Preparation time

under 70 minutes

Maximum loading volume15 ml
Binding capacity300 μg
Endotoxin level<0.1 EU/μg
Maximum Elution volume500 μl

Kit Contents

Plasmid EF Midi (Cat. No. 121-220 / 121-201)

  • Midi SV column type E 
  • EzClear™ Filter column (blue ring)
  • Collection tubes
  • Buffer P1
  • Buffer P2
  • Buffer P3
  • Buffer ER
  • Buffer EG
  • Buffer EW1
  • Buffer EW2
  • Buffer EF
  • RNase A (20 mg/ml)
  • Technical Data

        Transfection Efficiency

           

    Protocols

    Supplementary Protocols

    Resources

    Application Notes

    References