GeneAll Biotechnology Co., Ltd.

10 Agriculture: Sweet patato total RNA: Sweet potato (Ipomoea batatas L.) is a perennial plant belonging to Convolvulaceae Family and is regarded as one of the representative root vegetables. It is consumed as a food, but it is also utilized in a variety of industrial sectors, including processed food, pharmaceuticals, cosmetics, and bioenergy generation. Recently, it has attracted considerable attention due to its high potential and possibilities, particularly for utilization as a climate-resilient crop and in functional food development. Consequently, active research is being conducted to develop sweet potato varieties that exhibit excellent quality and high added value. In this research process, genome analysis and functional studies provide essential foundational data for breeding superior varieties and developing new cultivars.
The AllEx® Mini Plant Total RNA Kit and the AllEx® Plant Total RNA Kit have been developed for the efficient extraction of total RNA from a wide range of plant samples. These kits have been optimized for use with the AllEx® Mini Automated Nucleic Acid Extraction System and the AllEx®64 Automated Nucleic Acid Extraction System, respectively. The technology utilizes magnetic bead-based nucleic acid extraction. This method has been demonstrated to enable the rapid and reliable isolation of high-quality total RNA from a diverse range of plant species. The AllEx® Mini Automated Nucleic Acid Extraction System facilitates the concurrent processing of up to 12 samples, while the AllEx®64 Automated Nucleic Acid Extraction System is capable of accommodating up to 64 samples.
This Application Note describes the extraction of total RNA from the tuberous roots of sweet potato, a starch-enriched plant tissue that poses challenges for nucleic acid isolation, using the AllEx® Mini Plant Total RNA Kit and the AllEx® Plant Total RNA Kit. Optimal conditions for RNA extraction from sweet potato were established, and the concentration and purity of the isolated RNA were measured. Furthermore, electrophoresis analysis was performed to verify and evaluate the yield and quality of the extracted RNA.

Application Notes: Proposal of optimal sample amounts for high-quality total RNA extraction from sweet potato using the AllEx® Mini Plant Total RNA Kit and AllEx® Plant Total RNA Kit

Products: AllEx® Mini Automated Nucleic Acid Extraction System

AllEx® Mini Plant Total RNA Kit

AllEx®64 Automated Nucleic Acid Extraction System

AllEx® Plant Total RNA Kit

09 Agriculture: Seed DNA: Corn (Zea mays L.) is a representative annual crop belonging to monocotyledonous plants and is classified as one of the world’s three major cereals along with rice and wheat. In addition to being widely used as food and livestock feed, it also holds significant value in various fields such as biofuel and industrial raw materials. In modern times, driven by factors such as climate change, food security, and economic demand, research on developing corn varieties with superior quality and productivity has been actively conducted. In this process, genomic research and functional analysis of corn play a crucial role in the development of new varieties.
The AllEx® Mini Plant DNA Kit has been developed to efficiently extract genomic DNA from a wide range of plant samples and is optimized for use with the AllEx® Mini Automated Nucleic Acid Extraction System. Based on magnetic bead–based nucleic acid extraction technology, this kit enables rapid and reliable DNA extraction.
This Application Note describes the optimization of extraction protocols on the AllEx® Mini Automated Nucleic Acid Extraction System using the AllEx® Mini Plant DNA Kit to obtain high-yield, high-purity genomic DNA from dried corn seeds. DNA yield and purity obtained under different conditions were measured and evaluated, and the quality of the extracted DNA was verified by electrophoresis.

Application Notes: Optimized extraction protocols for high-yield and high-quality genomic DNA isolation from dried corn kernels using the AllEx® Mini Plant DNA Kit

Products: AllEx® Mini Automated Nucleic Acid Extraction System

AllEx® Mini Plant DNA Kit

08 Fishery: Bryophyte DNA: Bryophytes represent the initial group of plants to emerge, with approximately 20,000 species currently recognized worldwide and 900 species endemic to Republic of Korea. These plants have been utilized in urban greening initiatives within Japan and select European nations, owing to their capacity to absorb air pollutants and purify the atmosphere. Additionally, they are being regarded as potential pioneers in the realm of plant cultivation on Mars, a consideration that stems from their remarkable resilience to extreme conditions and radiation. Consequently, bryophytes are anticipated to possess substantial value as a biological resource in the future. The identification of undocumented species and the undertaking of taxonomic research are ongoing endeavors. In order to utilize bryophytes for genetic classification and various studies, it is imperative to extract high-quality nucleic acids.
The AllEx® Plant DNA/RNA Kit was developed to facilitate the extraction of total nucleic acids from a variety of plant samples. This kit is optimized for the AllEx ® 64 Automated Nucleic Acid Extraction System. The technology is predicated on the use of magnetic beads for the extraction of nucleic acids, a process that is rapid and efficient.
In this application note, we describe the extraction of nucleic acids from male and female individuals of Polytrichum commune and Marchantia polymorpha, representative species of Bryophyte, using the AllEx ® Plant DNA/RNA Kit, which is specialized for a variety of plant samples.

Application Notes: The performance evaluation of genomic DNA extraction using the AllEx® Plant DNA/RNA Kit from two bryophyte species (Polytrichum commune and Marchantia polymorpha)

Products: AllEx®64 Automated Nucleic Acid Extraction System

AllEx® Plant DNA/RNA Kit

07 Fishery: Halophyte DNA: In nature, there are many plants that live in unique environments that are different from the general environment. Among them, halophytes (e.g., Spergularia marina, Suaeda japonica, and Suaeda maritima) are plants that live in environments with salt, such as coastal wetlands, sand dunes, and salt pans. The criteria for classifying halophytes vary among taxonomist, such as whether they require salt, their habitat, or their physiological characteristics. They are mainly used as edible or medicinal resources in the food or pharmaceutical fields. In genetic research, accumulated salt and polysaccharides in halophytes are among the factors that significantly reduce nucleic acid extraction efficiency, making them one of the difficult materials to handle.
Exgene™ Plant SV is a product for genomic DNA extraction from various plant samples, and customers are able to choose mini, midi, and maxi scale following sample amount. This product is specialized for high-quality DNA extraction using EzSep™ Filter for efficient removing of polysaccharide and cell debris that reduce nucleic acid extraction efficiency in plants, and the purified DNA can be applied to various downstream applications.
The following study analyzes the genomic DNA extracted from Spergularia marina, type of halophyte, using Exgene™ Plant SV maxi based on yield, purity, and gel electrophoresis. In this experiment, we applied a customized protocol to remove the large amount of salt and polysaccharide in halophytes that lower the nucleic acid extraction efficiency. Also, we compared the yield and purity with those obtained by extracting DNA using standard protocol.

Application Notes: Genomic DNA extraction performance of customized protocol of Exgene™ Plant SV maxi from Halophyte (Spergularia marina)

Products: Exgene™ Plant SV maxi

06 Plant: Leaf DNA: Plants are known to be the most species distributed on Earth, and this diversity is most closely related to plant research. The plant research is difficult due to the specificity of each plant species especially in the field of molecular biology. Debris removal is essential at the nucleic acid extraction stage, and it is most important to remove it effectively. This application note is data obtained from high-quality gDNA from various plant leaves using the Exgene™ Plant SV mini kit, and please use it as a reference for plant research.

Application Notes: Exgene™ Plant SV is specialized for extracting high-quality genomic DNA from various plant leaves

Products: Exgene™ Plant SV

05 Agriculture: Fruit RNA: Damage to food resources is accelerating due to various effects such as pests that hinder plant growth. Research to improve varieties and produce healthy crops to prevent such damage and to produce eco-friendly crops is becoming important.This application note extracts total RNA with Ribospin™ Seed/Fruit kit from pulp samples of various fruits/fruits, and please use it as a reference for research.

Application Notes: Ribospin™ Seed/Fruit is a specialized kit that enables high-quality total RNA extraction from various fruit samples

Products: Ribospin™ Seed/Fruit

04 Agriculture: Seed RNA: The vitality of crops is largely controlled by various environmental factors (temperature, precipitation, sunlight, etc.). These environmental factors cannot be artificially controlled, so crop improvement is essential according to environmental changes. This application note is the result of extracting total RNA from various plant seed samples with Ribospin™ Seed/Fruit kit. Please use it as a reference for seed research.

Application Notes: Ribospin™ Seed/Fruit is a specialized kit that enables high-quality total RNA extraction from various seed samples

Products: Ribospin™ Seed/Fruit

03 Plant: Leaf RNA: As climate change accelerates, the plant research is becoming more active to preserve food resources. The extraction of highly concentrated /purified nucleic acids is essential for the research. This application note is the result of extracting total RNA from rice and potato leaf samples with Ribospin™ Seed/Fruit kit, and please use it as a reference for plant research.

Application Notes: Total RNA extraction from Oryza sativa leaf and Solanum tuberosum leaf using Ribospin™ Seed/Fruit

Products: Ribospin™ Seed/Fruit

02 Fishery: Sea algae RNA: Since sea algae is the primary producers of the marine ecosystem and is widely used as human edible material or bio-resource, many studies are being conducted. However, it is known that nucleic acid extraction is difficult because the salt contained in sea algae acts as an obstacle to extracting nucleic acids.
This application note is an experiment to confirm whether it is possible to extract total RNA from sea algae samples with Ribospin™ Plant kit, and please use it as a reference for sea algae research.

Application Notes: Total RNA extraction from Ulva linza Linnaeus samples using Ribospin™ Plant kit

Products: Ribospin™ Plant

01 Fishery: Sea algae DNA: Sea algae plays as a primary producer in the marine eco system and is widely used as nutritional and functional food sources or bio-resource. So many studies have been conducted, but salt contained in sea algae is known to hinder nucleic acid extraction, making it difficult to extract nucleic acids.This application note is the result of checking whether gDNA can be extracted from the seaweed sample with Exgene™ Plant SV mini kit, and please use it as a reference for seaweed research.

Application Notes: Genomic DNA extraction experiment using Exgene™ Plant SV mini kit from tiny amount of sea algae samples which are difficult to extract nucleic acid

Products: Exgene™ Plant SV

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