PRODUCTS
- PRODUCTS
- PLASMID DNA
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Endotoxins (lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli. It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. GeneAll® Exfection™ Plasmid LE provides simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.
Endotoxins (lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations.
GeneAll® Exfection™ Plasmid LE provides simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.
Exfection™ Plasmid LE utilizes glass microfiber membrane.
| Features | Plasmid LE mini | Plasmid LE Midi | ||||
|---|---|---|---|---|---|---|
| Format | Column Type Qe with collection tube | Column Type E with collection tube | ||||
| Recommended sample volume | 2~10 ml LB | 5~100 ml LB | ||||
| Maximum sample volume | Up to 10 ml LB | Up to 100 ml LB | ||||
| Lysate clearing | Centrifugation | EzClear™ Filter | ||||
| Preparation time | under 30 minutes | under 50 minutes | ||||
| Maximum loading volume | 800 μl | 15 ml | ||||
| Binding capacity | 30 μg | 300 μg | ||||
| Endotoxin level | <10 EU/μg | <10 EU/μg | ||||
| Maximum Elution volume | 50 μl | 500 μl | ||||
Kit Contents
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Transfection Efficiency |
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