GeneAll Biotechnology Co., Ltd.

04 Performance evaluation: cfDNA: Cell-free DNA (cfDNA) is DNA that circulates freely in blood and other body fluids, released from cells. First recognized in 1977, cfDNA levels were found to be higher in cancer patients than in healthy individuals. Since then, cfDNA has been widely used in diagnostics for both cancer and prenatal testing. In cancer, cfDNA helps track mutations and assess tumor heterogeneity. In prenatal testing, fetal cfDNA in maternal blood is analyzed to detect genetic abnormalities, providing a non-invasive alternative to traditional diagnostic methods.
Despite its utility, a major challenge is the low yield of cfDNA, which can limit downstream applications. To overcome this, the Exgene™ cfDNA SV mini was developed, optimized to efficiently isolate cfDNA without the need for organic solvents or pre-treatment.
This study aims to evaluate the performance of the Exgene™ cfDNA SV mini by comparing it with a competitor's equivalent kit in cancer patient plasma and serum samples.

Application Notes: Extraction of cfDNA from cancer plasma & cancer serum using Exgene™ cfDNA SV mini and detection of specific genes

03 Performance evaluation: Blood DNA: Blood is main samples in the field of life research and diagnostic testing. The blood is composed of a variety of components which enable to sustain life activities, and buffy coat accounting for less than 1% of blood is comprised of white blood cells and platelets. White blood cells are produced from bone marrow to protect the body against external substances. Whenever wound incurred, platelets get together to this part to stop bleeding through blood coagulation.
While other components such as hematin, hemoglobin, plasma proteins, and other substances act as inhibitors. Therefore, they must be removed thoroughly during the nucleic acid extraction step and it is key point for successful research and diagnostic testing.
GenEx™ Blood Sx serves 'Special Buffer System' to extract high-quality gDNA from various blood samples and remove inhibitors. The quantification (yield, purity) and real-time PCR was carried out on extracted gDNA using GenEx™ Blood Sx to evaluate performance.

Application Notes: Genomic DNA extraction with GenEx™ Blood from human whole blood and buffy coat (PBMC) isolated from human whole blood

02 Performance evaluation: Blood DNA: Blood is vitally important fluid for the body to transports necessary substances such as oxygen and nutrients to all the parts of the body. Blood, as an ideal source of pathogen detection, forensic identification, disease diagnosis, immunoassays, and other applications, the research on blood has been in constant demand, especially if they utilize less-invasive blood extractions and are coupled with sensitive applications.
The purpose of this application is to evaluate the performance of GENTi™ Advanced Blood DNA Extraction Kit in combination with GENTi™ Advanced Automated Nucleic Acid Extraction Equipment for the extraction of four different blood specimen types.

Application Notes: Blood gDNA Extraction from Various Blood Specimen Types using GENTi™ Advanced Blood DNA Extraction Kit

Products: GENTi™ Advanced Blood DNA Extraction Kit

GENTi™ 32 Advacned Automatic Extraction Equipment

01 Forensic samples: Genomic DNA: Forensic DNA analysis is a powerful tool in criminal investigations and legal proceedings. It involves the extraction of DNA from various types of forensic samples, including blood stains, hair, cigarette butts, fingerprints, and saliva. These samples are collected from crime scenes and can be used to identify suspects and provide evidence for criminal cases. The use of forensic DNA analysis has expanded beyond criminal investigations, and it is now commonly used in various fields, such as drug testing, medicine, food inspection, and accident or missing person identification.
The extracted DNA is subjected to various analysis techniques, such as polymerase chain reaction (PCR) which allows for the amplification of very small amounts of DNA that may be present in a forensic sample.
This application note assessed the efficiency of GENTi™ Advanced Blood DNA Extraction Kit in extracting DNA from micro forensic samples using Real-time PCR.

Application Notes: Genomic DNA Extraction from Diverse Forensic Samples using GENTi™ Advanced Blood DNA Extraction Kit on GENTi™ 32 Advanced Automatic Extraction System

Products: GENTi™ Advanced Blood DNA Extraction Kit

GENTi™ 32 Advacned Automatic Extraction Equipment

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