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Exprep™ Plasmid SV

GeneAll® Exprep™ Plasmid SV provides a rapid and convenient method for the small and medium scale preparations of plasmid DNA from bacterial cells and it is used to isolate and purify any plasmids from any E. coli strains. Exprep™ Plasmid SV can yield up to 30 μg (mini) of highly purified plasmid DNA and it can be applicable directly for PCR, cloning, automated sequencing, synthesis of labeled hybridization probes and other enzymatic reactions without further manipulation.

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Overview

GeneAll® Exprep™ Plasmid SV provides a rapid and convenient method for the small and medium scale preparations of plasmid DNA 

from bacterial cells and it is used to isolate and purify any plasmids from any E. coli strains. 

Exprep™ Plasmid SV  can yield up to 30 μg (mini) of highly purified plasmid DNA and it can be applicable directly for PCR, 

cloning, automated sequencing, synthesis of labeled hybridization probes and other enzymatic reactions without further manipulation.

01

Format

Exprep™ Plasmid SV utilizes glass microfiber membrane based on the modified alkaline lysis method.

  • Spin column format
  • Cell debris and salt precipitates are removed by centrifugation for mini kit and by EzClear™ Filter column for Midi kit
  • Midi kits require the centrifuge which has swing bucket rotor and ability of 4,000 x g at least.
02

High purity

  • Stable and consistent result
  • High yield and purity
03

Simple and safe procedure

  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Suitable for PCR, cloning, fluorescent sequencing, synthesis of labeled hybridization probes, cell transfection, electroporation, and enzymatic restriction analysis without further manipulation

specifications

Technical Information

Features Plasmid SV mini Plasmid SV Midi
Format Column type Q (mini) with collection tubeColumn type Q (Midi) with collection tube
Recommended sample volume5 ml LB50 ml LB
Maximum sample volumeUp to 10 ml LBUp to 100 ml LB
Lysate clearingCentrifugationEzClear™ Filter
Preparation timeunder 23 minutes

under 50 minutes

Maximum loading volume800 μl
15 ml
Binding capacity30 μg300 μg
Recovery rate85~95%85~95%
Maximum Elution volume40 μl400 μl

Kit Contents

Plasmid SV mini

(Cat. No. 101-150 / 101-102)

Plasmid SV Midi

(Cat. No. 101-226 / 101-201)

  • mini SV column type Q
  • Collection tubes
  • Buffer S1
  • Buffer S2
  • Buffer S3
  • Buffer AW (concentrate)
  • Buffer PW concentrate)
  • Buffer EB
  • RNase A (20 mg/ml)
  • Midi SV column type Q (red ring)
  • EzClear™ Filter column (blue ring)
  • Collection tubes
  • Buffer S1
  • Buffer S2
  • Buffer S3
  • Buffer AW (concentrate)
  • Buffer PW concentrate)
  • Buffer EB
  • RNase A (20 mg/ml)
  • Technical Data

        Stable and Reproducible Result 

      

        DNA Automated Squencing Analysis

        Compatibility Test with Restriction Enzymes 

    Protocols

    Supplementary Protocols