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AmpONE™ Taq DNA Polymerase

AmpONE™ Taq DNA Polymerase는 Thermus aquaticus에서 유도된 재조합 효소로, 일반 PCR, DNA sequencing, TA cloning 등 다양한 실험에 사용할 수 있습니다.

SCALE / SIZE

Overview

AmpONE™ Taq DNA Polymerase는 Thermus aquaticus에서 유도된 재조합 효소로, 일반 PCR, DNA sequencing, TA cloning 등 다양한 실험에 사용할 수 있습니다.

01

Description

  • Recombinant enzyme derived from Thermus aquaticus, which is cloned and expressed in Escherichia coli and possesses the same functions as the native enzyme.
02

High purity

  • No contamination of genomic DNA
  • Suitable for the amplification of fragments up to 5 kb from template.
03

3' end A-tailing 형성

  • 5’→3’ polymerase activity
  • No 3’→5’ exonuclease (proofreading) activity
04

Applications

  • Suitable for various experiments such as conventional PCR, DNA sequencing and TA cloning.

specifications

Technical Information

Features Specifications
Unit definition One unit is the amount of AmpONETM Taq DNA Polymerase required to incorporate 10 nmol of dNTP into acid-insoluble product in 30 minutes at 72°C.
Synthesis efficiency ~5 kb
Exonuclease activity None detected
Endonuclease activity None detected
Storage condition Stable for 18 months at -20°C

Kit Contents

 

Cat. No. 501-025

(250 units)

Cat. No. 501-050

(500 units)

Cat. No. 501-100

(1,000 units)
Taq DNA Polymerase 50 μl x 1 tube
50 μl x 2 tubes50 μl x 4 tubes
10X Reaction Buffer
1.0 ml x 1 tube
1.0 ml x 2 tubes
1.0 ml x 4 tubes
2.5 mM dNTP Mix0.8 ml x 1 tube

0.8 ml x 2 tubes

0.8 ml x 4 tubes

6X Loading Dye
1.0 ml x 2 tubes
1.0 ml x 4 tubes
1.0 ml x 8 tubes

Technical Data

Protocols

Handbooks

Supplementary Protocols