HyperScript™One-Step RT-PCR Master Mix is a 2X premix ready to use for Reverse Transcriptase(RT) reaction and Polymerase Chain Reaction(PCR). This mix contains HyperScript™ reverse transcriptase and AmpONE™ HS-Taq DNA polymerase and both RT and PCR reactions are carried out successively in a single tube. HS-Taq polymerase can amplify the fragment up to 5 kb in length.
HyperScript™ One-Step RT-PCR Master mix is a 2X premix ready to use for Reverse Transcriptase(RT) reaction and Polymerase Chain Reaction(PCR).
This mix contains HyperScript™ reverse transcriptase and AmpONE™ HS-Taq DNA polymerase and
both RT and PCR reactions are carried out successively in a single tube. HS-Taq polymerase can amplify the fragment up to 5 kb in length.
Features | Specifications |
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Unit definition | One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 min at 37°C using poly(A) · oligo(dT)20 as template- primer. |
Synthesis efficiency | ~5 kb |
Storage condition | Stable for 1 year at -20°C |
HyperScript™ One-Step RT-PCR Master Mix (Cat. No. 602-110) |
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contains HyperScript™ reverse transcriptase, HS-Taq DNA polymerase, reaction buffer, dNTPs, RNase inhibitor and stabilizer. |