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Ribospin™ II

GeneAll® Ribospin™ II is devised to purify RNA from cultured cells or animal tissues. With the GeneAll’s glassfiber membrane technology, highly pure RNA can be conveniently isolated in less than 30 minutes instead of the time consuming and hazardous conventional methods which require alcohol precipitation or toxic chemicals such as phenol / chloroform. The optimized buffer system of Ribospin™ II maximizes the specific binding efficiency of RNA to the glassfiber membrane but minimizes the contamination of impurities by a series of optimized wash buffer. Also, the contaminated DNA residues can be easily eliminated during the preparation by on-column digestion using DNase I included in this kit.

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Overview

GeneAll® Ribospin™ II is devised to purify RNA from cultured cells or animal tissues. 

With the GeneAll’s glassfiber membrane technology, highly pure RNA can be conveniently isolated in less than 30 minutes instead of the time

consuming and hazardous conventional methods which require alcohol precipitation or toxic chemicals such as phenol / chloroform. 

The optimized buffer system of Ribospin™ II maximizes the specific binding efficiency of RNA to the glassfiber

membrane but minimizes the contamination of impurities by a series of optimized wash buffer. Also, the contaminated DNA

residues can be easily eliminated during the preparation by on-column digestion using DNase I included in this kit. 

01

Format

Ribospin™ II utilizes glassfiber membrane technology for the isolation of total RNA.

  • Spin column format
02

High purity and consistent yield

  • Upgraded lysis buffer system, excellent lysis power and minimized bubble formation
  • The contaminated DNA residues can be easily eliminated during the preparation by on-column digestion using DNase I
03

Simple and safe procedure

  • Preparation time : ~30 minutes
  • No phenol/chloroform extraction
  • No alcohol precipitation
04

Applications

  • Ready for use in cloning, automated sequencing, in vitro transcription,labeling, microarray, hybridization and other enzymatic reactions

specifications

Technical Information

Features Ribospin™ II
Format Column Type F with collection tube
Maximum amount of starting samples ~30 mg tissue or ~1 x 107 cells
Maximum loading volume ~750 μl
Minimum elution volume ~30 μl
Binding capacity ~500 μg

Kit Contents

Ribospin™ II (Cat. No. 314-150 / 314-103)

  • Column type F 
  • Collection tubes
  • Buffer RAL
  • Buffer RW
  • Buffer RSW (concentrate)
  • Nuclease-free water
  • BUffer DRB
  • DNase I
  • Technical Data

        Total RNA Isolation from CHO and rat liver cell Using RibospinTM II

           

        Comparison Data - Real time PCR amplificaton and cDNA synthesis.

           
           

    Protocols

    Supplementary Protocols