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Exgene™ FFPE Tissue DNA

GeneAll® Exgene™ FFPE Tissue DNA provides a convenient and easy method for the isolation of total DNA from Formalin Fixed and Paraffin Embedded (FFPE) specimen by non-organic solvent. FFPE is one of the most commonly used methods of clinical tissue preservation; the clinical tissue is fixed by formalin and subsequently embedded in paraffin to keep its original form. The FFPE tissue is useful in disease research such as microscopic observation and immunohistochemical analysis. And the extracted nucleic acid from FFPE specimen can be used for molecular diagnosis of various diseases. Purified DNA can be used directly for PCR(≤500 bp), real-time PCR, and other downstream applications.

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Overview

GeneAll® Exgene™ FFPE Tissue DNA provides a convenient and easy method for the isolation of total DNA from Formalin Fixed and 

Paraffin Embedded (FFPE) specimen by non-organic solvent. FFPE is one of the most commonly used methods of 

clinical tissue preservation; the clinical tissue is fixed by formalin and subsequently embedded in paraffin to keep its original form. 

The FFPE tissue is useful in disease research such as microscopic observation and immunohistochemical analysis. 

And the extracted nucleic acid from FFPE specimen can be used for molecular diagnosis of various diseases. 

Purified DNA can be used directly for PCR(≤500 bp), real-time PCR, and other downstream applications. 

01

Format

Exgene™ FFPE Tissue DNA utilizes the advanced silica-binding technology.

  • Spin column format
02

Stable and consistent result

  • Guaranteed PCR product length up to 500 base pair
  • RNase A included for pure DNA
03

Simple and safe procedure

  • Use of non-xylene based Buffer DP
  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Can be used directly for PCR (≤500 bp), real-time PCR, and other downstream applications.

specifications

Technical Information

Features Specifications
Format Column type G with collection tube
Maximum amount of starting samples
Up to 8 sections of 10 μm in thickness
Maximum loading volume ~750 μl
Elution volume ~30 μl
Maximum binding capacity ~60 μg

Kit Contents

Exgene™ FFPE Tissue DNA (Cat. No. 138-150 / 138-152)

  • mini column type G
  • Collection tubes
  • Buffer DP
  • Buffer FPL
  • Buffer FPB 
  • Buffer BW (concentrate)
  • Buffer TW (concentrate)
  • Buffer AE
  • RNase A (100 mg/ml)
  • Proteinase K
  • PK storage buffer
  • Technical Data

       Comparative Data - PCR Amplification Test I

               

        Comparative Data - PCR Amplification Test II

                 

        Comparative Data - Real-Time PCR Amplification Test

                 

    Protocols

    Supplementary Protocols

    Resources

    Application Notes