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Exgene™ Cell SV

GeneAll® Exgene™ Cell SV provides a rapid and simple method for the purification of total DNA from a wide range of organism including bacterial cells, yeast, cultured cells, whole blood and blood derivatives. The pure DNA can be acquired in just 30 minutes and this can be directly used in various applications such as PCR, Southern blotting and other enzymatic reactions.

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Overview

GeneAll® Exgene™ Cell SV provides a rapid and simple method for the purification of total DNA from a wide range of organism 

including bacterial cells, yeast, cultured cells, whole blood and blood derivatives. The pure DNA can be acquired in just 30 minutes and 

this can be directly used in various applications such as PCR, Southern blotting and other enzymatic reactions.

01

Format

Exgene™ Cell SV utilizes glass microfiber membrane.

  • Spin column format
  • MAXI kits require the centrifuge which has swing bucket rotor and ability of 4,000 x g at least.
02

High purity

  • Stable and consistent DNA extraction from gram positive or negative bacteria, cultured cell, yeast and various biological samples
  • High purity : A260/A280 = 1.8~2.0
03

Simple and safe procedure

  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions.

specifications

Technical Information

Features Cell SV mini Cell SV MAXI
Format Column type G (mini) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size
 ~2 x 109 bacterial cells
~5 x 107 yeast cells
~5 x 1010 bacterial cells
~5 x 108 yeast cells
Preparation time30~120 minutes

60~240 minutes 

Typical yield5~25 μg80~400 μg
Elution volume30~400 μl0.4~2 ml

Kit Contents

Cell SV mini

(Cat. No. 106-101 / 106-152)

Cell SV MAXI

(Cat. No. 106-310 / 106-326)

  • mini column type G
  • Collection tubes
  • Buffer GP
  • Buffer YL
  • Buffer CL
  • Buffer BL
  • Buffer BW (concentrate)
  • Buffer TW (concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
  • MAXI column type G
  • Collection tubes
  • Buffer GP
  • Buffer YL
  • Buffer CL
  • Buffer BL
  • Buffer BW (concentrate)
  • Buffer TW (concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
  • Technical Data

        Consistent Result from Various Samples

           

        Compatibility Test with Restriction Enzymes

           

        PCR Amplification Test

           

    Resources