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Exgene™ Tissue SV Plus

GeneAll® Exgene™ Tissue SV Plus provides a simple and rapid method for the isolation of total DNA from animal tissues, cultured cells, whole blood and its derivatives. This kit can process 25 mg (mini) of wet tissue and yields up to 50 μg (mini) depending on the type of sample used.

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Overview

GeneAll® Exgene™ Tissue SV Plus provides a simple and rapid method for the isolation of total DNA from animal tissues, cultured cells, 

whole blood and its derivatives. This kit can process 25 mg (mini) of wet tissue and yields up to 50 μg (mini) depending on the type of sample used.

01

Format

Exgene™ Tissue SV Plus utilizes glass microfiber membrane.

  • Spin column format
  • Midi and MAXI kits require the centrifuge which has swing bucket rotor and ability of 4,000 x g at least.
02

High purity

  • Stable and consistent result
  • High yield and purity : A260 / A280 = 1.8~2.0
03

Simple and safe procedure

  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Ready for use in PCR, Southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions.

specifications

Technical Information

Features Tissue SV Plus mini Tissue SV Plus Midi Tissue SV Plus MAXI
Format Column type G (mini) with collection tubeColumn type G (Midi) with collection tubeColumn type G (MAXI) with collection tube
Maximum sample size~25 mg~100 mg~250 mg
Preparation time25~220 minutes

40~250 minutes 

40~250 minutes 

Maximum loading volume750 μl5 ml16 ml
Binding capacity60 μg200 μg600 μg
Elution volume30~400 μl200~600 μl0.4~2 ml

Kit Contents

Tissue SV Plus mini

(Cat. No. 109-101 / 109-152)

Tissue SV Plus Midi

(Cat. No. 109-226 / 109-201)

Tissue SV Plus MAXI

(Cat. No. 109-310 / 109-326)
  • mini column type G
  • Collection tubes
  • Buffer RL
  • Buffer TL
  • Buffer TB
  • Buffer BW (concentrate)
  • Buffer TW concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
    		•
  • Midi column type G
  • Collection tubes
  • Buffer RL
  • Buffer TL
  • Buffer TB
  • Buffer BW (concentrate)
  • Buffer TW concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
    		•
  • MAXI column type G
  • Collection tubes
  • Buffer RL
  • Buffer TL
  • Buffer TB
  • Buffer BW (concentrate)
  • Buffer TW concentrate)
  • Buffer AE
  • Proteinase K
  • PK Storage buffer
    		•

    Technical Data

        Maximum amount of starting sample

       

        Genomic DNA Extraction from Various Samples

       

    Protocols

    Handbooks

    Supplementary Protocols