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Exgene™ Stool-Bead DNA

GeneAll® Exgene™ Stool-Bead DNA provides a convenient method for the isolation of total DNA from stool samples. This kit utilizes a double binding procedure using the optimized buffer system, the advanced silica binding technology and newly developed glass bead beating technology to purify nucleic acid suitable for many applications. Through this method, the contained impurities in the starting stool samples are removed so that high quality DNA can be purified from host and microbial cells. The stool samples can be applied up to 200 mg per prep and this procedure can be completed in 25 minutes.

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Overview

GeneAll® Exgene™ Stool-Bead DNA provides a convenient method for the isolation of total DNA from stool samples. 

This kit utilizes a double binding procedure using the optimized buffer system, the advanced silica binding technology and
newly developed glass bead beating technology to purify nucleic acid suitable for many applications.

Through this method, the contained impurities in the starting stool samples are removed so that high quality DNA can be purified from host and microbial cells. 

The stool samples can be applied up to 200 mg per prep and this procedure can be completed in 25 minutes.

 

01

Format

Exgene™ Stool-Bead DNA utilizes glassfiber membrane technology for the isolation of total DNA.

  • Spin column format
02

Stable and consistent yield

  • Purification of high-quality DNA by the use of glass bead beating technology
03

Simple and safe procedure

  • Preparation time : ~25 minutes
  • No phenol / chloroform extraction
  • No alcohol precipitation
04

Applications


  • Ready for use in conventional PCR, electrophoresis, Real-time PCR, NGS, Genotyping, restriction analysis, and any other downstream applications.

specifications

Technical Information

Features Exgene™ Stool-Bead DNA 
Format Column Type G with collection tube
Maximum amount of starting samples ~200 mg stool sample
Maximum loading volume ~750 μl
Minimum elution volume30 μl
Binding capacity ~100 μg
Preparation time ~25 minutes

Kit Contents

Exgene™ Stool-Bead DNA (Cat. No. 115-151)
  • Column type G mini
  • 2 ml Glass Bead tubes
  • Collection tubes
  • Buffer FL
  • Buffer PB
  • Buffer KW30
  • Buffer NW (concentrate)
  • Buffer EB
  • Proteinase K (24 mg)
  • Proteinase K Storage Buffer

    • Technical Data

        Comparative Data 1   Real-Time PCR Amplification Test

                
      

    Figure 1. Results from salmonella infection clinical specimens :
    Real-time PCR analysis was conducted to amplify isolated DNA by Exgene™ Stool-Bead DNA (Blue) and Exgene™ Stool DNA mini (Red). Two repeat tests were performed for each sample.

        Comparative Data 2   Real-Time PCR Amplification Test

                
      

    Figure 2. Results from mycoplasma spiking swine stool sample :
    Real-time PCR analysis was conducted to amplify isolated DNA by Exgene™ Stool-Bead DNA (Blue) and Exgene™ Stool DNA mini (Gray).

        Comparative Data 3   Real-Time PCR Amplification Test

                
      

    Figure 3. Results from normal swine stool sample :
    Real-time PCR analysis was conducted targeting swine GAPDH gene. Genomic DNA was extracted by Exgene™ Stool-Bead DNA (Green) and Exgene™ Stool DNA mini (Red). Two repeat tests were performed for each sample.

    Protocols

    Handbooks

    Supplementary Protocols