GeneAll Biotechnology Co., Ltd.

Overview

GeneAll® Ribospin™ Plant는 잎, 줄기, 뿌리와 까다로운 식물 샘플을 포함하는 다양한 식물 조직에서 전체 RNA를 정제할 수 있는 제품입니다.

이 키트는 다당류 및 폴리 페놀 화합물을 제거하고 온전한 식물 RNA를 효과적으로 분리하기 위해 최적화된 버퍼와 스핀 컬럼을 제공합니다.

01

Format

Advanced silica-based nucleic acid purification
Most impurities except RNA in the lysate are eliminated by filtration through EzPure™ Filter

02

Consistent yield and high purity

High purity
A₂₆₀/A₂₈₀ = 1.8–2.2
A₂₆₀/A₂₃₀ > 2.0

03

Simple and safe procedure

Preparation time: Up to 25 minutes
No phenol/chloroform extractions
No alcohol precipitation

04

Applications

Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assay and other analytical procedures.

specifications

Technical Information

Specifications

Ribospin™ Plant

Format

Column type W Mini

Maximum amount of starting samples

Up to 100 mg plant tissue

Maximum loading volume of EzPure™ Filter Mini

Up to 600 μl

Maximum loading volume of Column type W Mini

Up to 700 μl

Minimum elution volume

30 μl

Maximum binding capacity

Up to 100 μg

Storage conditions

Store at room temperature (15 ℃ to 25 ℃)

Kit Contents

Product	Ribospin™ Plant
Cat. No.	307-106	307-150
Type	Mini	Mini
Number of preparation	6 *	50
Components	Quantity
Column type W Mini	6	50
EzPure™ Filter Mini	12	100
1.5 ml microcentrifuge tube	6	50
Buffer RPL	3 ml	25 ml
Buffer REL	3 ml	25 ml
Buffer RBW **	3 ml	27 ml
Buffer RNW **	2 ml	12 ml
Nuclease-free water	1.5 ml	15 ml
DNase I	28 KU ***	240 KU ***
Buffer DRB	500 μl	5 ml
Kit manual	1	1
* Sample: free for testing Concentrated: absolute ethanol required * KU: Kunitz Units

Technical Data

Total RNA Yield from Various Starting Materials Using Ribospin™ Plant

RNA Purification Results

Comparison of Concentration and Integrity

RNA extracted from plant tissues using Ribospin™ Plant demonstrated excellent quality. Ribospin™ Plant achieved the high concentration and integrity, with RIN values reaching up to 9 (Embryo). This highlights Ribospin™ Plant as a reliable choice for extracting RNA of superior quality, suitable for downstream applications requiring high-purity and intact RNA.