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Ribospin™ Seed / Fruit

GeneAll® Ribospin™ Seed / Fruit is designed for easy and convenient isolation of total RNA from difficult plant tissues such as seeds, fruits, and rhizomes. Especially, this kit can remove effectively large quantities of secondary metabolites including polysaccharides and polyphenolic compounds which can lead to inhibition of downstream application. For efficient RNA purification, this kit offers optimized lysis system according to the sample type and adopts EzPure™ Filter to eliminate impurities simply from lysate. Moreover, contamination of genomic DNA, that causes interference in RNA analysis, can be excluded by on-column DNase I treatment in these procedures.

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Overview

GeneAll® Ribospin™ Seed / Fruit is designed for easy and convenient isolation of total RNA from difficult plant tissues such as seeds,

fruits, and rhizomes. Especially, this kit can remove effectively large quantities of secondary metabolites including polysaccharides

and polyphenolic compounds which can lead to inhibition of downstream application. For efficient RNA purification, this kit offers optimized lysis system according to the sample type and adopts EzPure™ Filter to eliminate impurities simply from lysate. Moreover, contamination of genomic DNA, that causes interference in RNA analysis, can be excluded by on-column DNase I treatment in these procedures.

01

Format

Ribospin™ Seed / Fruit utilizes glassfiber membrane technology for the fastest and the most convenient of high purity RNA isolation from plant samples.

  • Simply Removal of the impurities by using EzPure™ Filter
02

Consistent yield and high purity

  • A260/A280 = 1.8 ~ 2.2, A260/A230 > 2.0
03

Simple and safe procedure

  • Preparation time : ~30 minutes
  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Ready for use in RT-PCR, northern blotting, dot blotting, in vitro translation, molecular cloning, real-time PCR, RNase protection assay and other analytical procedures.

specifications

Technical Information

Features Ribospin™ Seed / Fruit
Format Column Type F with collection tube
EzPureTM Filter with collection tube
Maximum amount of starting samples ~100 mg / prep
Maximum loading volume of mini column ~750 μl
Minimum elution volume
30 μl
Preparation time ~30 minutes

Kit Contents

Ribospin™ Seed / Fruit (Cat. No. 317-150)

  • Column type F
  • EzPureTM Filter (yellow)
  • Collection tubes
  • Buffer SL
  • Buffer ML
  • Buffer RBW (concentrate)
  • Buffer RNW (concentrate)
  • Buffer DRB
  • DNase I
  • Nuclease free water
  • Technical Data

        Total RNA Yield from Several Kinds of Seeds Using RibospinTM Seed / Fruit

           

        Amplication test for CGMMV detection from infected seeds.

           

        Comparative Data for Total RNA Isolation

           

    Protocols

    Supplementary Protocols

    Resources