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Exgene™ Plant SV

GeneAll® Exgene™ Plant SV는 다양한 식물 조직에서 total DNA 정제를 위한 간단하고 쉬운 방법을 제공합니다. EzSep™ Filter와 Column Type G를 사용하면 40분 (최소) 이내에 정제 절차를 수행할 수 있으며, 정제된 genomic DNA는 추가 조작 없이 다양한 응용 실험에 사용 가능합니다.

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Overview

GeneAll® Exgene™ Plant SV는 다양한 식물 조직에서 total DNA 정제를 위한 간단하고 쉬운 방법을 제공합니다. 

EzSep™ Filter와 Column Type G를 사용하면 40분 (최소) 이내에 정제 절차를 수행할 수 있으며, 

정제된 genomic DNA는 추가 조작 없이 다양한 응용 실험에 사용 가능합니다.

01

Format

  • Advanced silica-based nucleic acid purification
  • Spin column format
  • Midi와 Maxi 제품 사용 시, 4,000 x g 이상의 swinging-bucket rotor를 장착한 원심분리기가 필요합니다.
02

High purity

  • Stable and consistent result
  • Perfect removal of second metabolites such as polyphenols and polysaccharides
03

Simple and safe procedure

  • Simple procedure by the use of EzSep™ Filter
  • No phenol/chloroform extractions
  • No alcohol precipitation
04

Applications

  • Ready for use in PCR, southern blotting, AFLP, RFLP, RAPD and other enzymatic reactions.

specifications

Technical Information

Specifications
Exgene™ Plant SV Mini Exgene™ Plant SV Midi Exgene™ Plant SV Maxi
Format Column type G Mini Column type G Midi Column type G Maxi
Maximum sample size Up to 100 mg wet
(Up to 25 mg dry)
Up to 400 mg wet
(Up to 100 mg dry)
Up to 1 g wet
(Up to 250 mg dry)
Preparation time <40 minutes <60 minutes <60 minutes
Maximum loading volume 750 μl 5 ml 15 ml
Typical yield 4–40 μg 10–150 μg 40–300 μg
Elution volume 30–400 μl 200–600 μl 0.4–2 ml
Storage conditions Store at room temperature (15 ℃ to 25 ℃)

Kit Contents

Product Exgene™ Plant SV Mini
Cat. No. 117-110 117-101 117-152
Type Mini Mini Mini
Number of preparation 10 * 100 250
Components Quantity
Column type G Mini 10 100 250
EzSep™ Filter Mini 10 100 250
Buffer PL 15 ml 100 ml 200 ml
Buffer PD 6 ml 30 ml 90 ml
Buffer BD ** 4 ml 37 ml 2 x 51 ml
Buffer CW ** 3 ml 30 ml 2 x 40 ml
Buffer AE 3 ml 60 ml 120 ml
RNase A (100 mg/ml) 48 μl 480 μl 1.3 ml
Kit manual 1 1 1
* Sample: free for testing
** Concentrated: absolute ethanol required

Product Exgene™ Plant SV Midi
Cat. No. 117-204 117-226 117-201
Type Midi Midi Midi
Number of preparation 4 * 26 104
Components Quantity
Column type G Midi 4 26 104
EzSep™ Filter Midi 4 26 104
Buffer PL 15 ml 100 ml 4 x 100 ml
Buffer PD 6 ml 30 ml 4 x 30 ml
Buffer BD ** 8 ml 37 ml 4 x 37 ml
Buffer CW ** 11 ml 50 ml 4 x 50 ml
Buffer AE 3 ml 60 ml 4 x 60 ml
RNase A (100 mg/ml) 80 μl 480 μl 4 x 480 μl
Kit manual 1 1 1
* Sample: free for testing
** Concentrated: absolute ethanol required

Product Exgene™ Plant SV Maxi
Cat. No. 117-302 117-310 117-326
Type Maxi Maxi Maxi
Number of preparation 2 * 10 26
Components Quantity
Column type G Maxi 2 10 26
EzSep™ Filter Maxi 2 10 26
Buffer PL 15 ml 100 ml 200 ml
Buffer PD 6 ml 30 ml 90 ml
Buffer BD ** 8 ml 37 ml 2 x 51 ml
Buffer CW ** 11 ml 50 ml 3 x 50 ml
Buffer AE 3 ml 60 ml 120 ml
RNase A (100 mg/ml) 80 μl 480 μl 1.3 ml
Kit manual 1 1 1
* Sample: free for testing
** Concentrated: absolute ethanol required

Technical Data

    Comparison of Genomic DNA Extraction

           
 

 

    Compatibility Test with Restriction Enzymes

           
 

 

    PCR Amplification Test

           

Addtional Information

YouTube - Exgene™ Plant SV for total DNA extraction from various plant cells, tissues, or fungi

 

Protocols

Supplementary Protocols

Resources