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GENESTATM > DNA Size Marker

제품명 GENESTATM 1 Kb DNA Ladder
브랜드 GeneAll
보관 -20 ℃
Cat.No.   Product Description Size 장바구니/견적요청/기타문의
GA-100 GENESTATM 1 Kb DNA Ladder 500 ul      
GA-010 GENESTATM 100 bp DNA Ladder 500 ul      
GA-025 GENESTATM 250 bp DNA Ladder 500 ul      

 

  • Standard Marker 가격에 더욱 세분화된 Reference Band를 가진 Plus Ladder 제공 !
  • 100bp Ladder의 경우 15개의 Size 구분 가능
  • 기준이 되는 Band의 Intensity를 높여 관찰의 편의성 제공 !
  • Loading Dye 500 ul 까지 공짜로 제공 !
  • Mupid Small Agarose Gel 기준, 500번 사용 가능 !
  • GENESTATM 하나면 Loading 준비 끝~ !

       

 

Description :

The 1 Kb DNA Ladder is designed and provided as molecular weight standards for agarose gel electrophoresis. The ladder consists of 13 fragments ranging in length from 250 to 12000 bp. The 1500 bp and 4000 bp bands have increased intensity to serve as reference bands. The approximate mass for each band is 50 ng DNA, and 150 ng for reference band, in the 5 ul sample.

The ladder is dissolved in 1x loading dye and ready-to-use.
5x Loading Dye is supplied as a free gift.


Storage / Stability :

Store at -20℃. The product is stable for 1 year stored at -20℃. It can be stored at least 3 months at 4℃.
Do not heat before loading.


Protocol :

1. Load 5 ul ladder for a gel lane, and adjust loading volumeaccording to the width of the agarose gel.
2. Use 0.7 - 1.2 % agarose, and 4 - 10 v/cm, in 1xTAE electrophoresis buffer.
3. Visualize DNA by EB (ethidium bromide) staining, or SYBR Green I.

 5x Loading Dye
    10 mM Tris - HCl,
    5 mM EDTA, pH7.6,
    0.03 % Bromophenol blue,
    0.03 % Xylene cyanol,
    30 % Glycerol.