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Oligonucleotide Synthesis > Molecular Beacons

제품명 Molecular Beacons
브랜드 Sigma Proligo
보관 -20 ° C
Cat.No.   Product Description Size 장바구니/견적요청/기타문의
Molecular Beacons      

 

Choose Molecular Beacons for:

  • SNP detection
  • Allele discrimination
  • Pathogen detection
  • Multiplexing
  • Viral load quantification
  • Gene expression analysis
  • Gene copy determination
  • End point genotyping
  • in vitro quantification or detection

Benefits of Using Molecular Beacons include:

  • Increased specificity
  • Probe preserved during the reaction

Add LNA to Your Probe for:

  • Increased thermal stability and hybridization specificity
  • Greater accuracy in SNP detection and allele discrimination
  • Easier and more sensitive probe designs for problematic target sequences

How Molecular Beacons Work

A Molecular Beacon is a single-stranded bi-labeled fluorescent probe held in a hairpin-loop conformation (around 20 to 25 nt) by complementary stem sequences (around 4 to 6 nt) at both ends of the probe. The 5' and 3' ends of the probe contain a reporter dye and a quencher molecule, respectively. The loop is a single-stranded DNA sequence complementary to the target sequence. The close proximity of the reporter and quencher causes the quenching of the natural fluorescence emission of the reporter dye. The structure and mechanism of a Molecular Beacon is shown below.

Molecular Beacons hybridize to their specific target sequence causing the hairpin-loop structure to open and separate the 5' end reporter dye from the 3' end quencher. As the quencher is no longer in proximity to the reporter dye, fluorescence emission takes place. The measured fluorescence signal is directly proportional to the amount of target DNA.


Product Features include:

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Custom formats available (normalizations, special plates, etc.)

Guaranteed Yields
Guaranteed
OD Yield
Approx. No.
of nmoles
Approx. No.
of µg
Approx. No.
of Reactions*
1 3 32 600
3 9 96 1,800
5 15 160 3,000
10 30 320 6,000
*Estimate is based on 3 nmoles or 32 µg for 1 OD and 200 nM in 25 µl reaction (5.0 pmol/reaction). Estimate is based on an average sequence length of 30 bases.

The most common fluorophore and quencher combinations are listed below:

Dye Max.
EX (nm)
Max.
EM (nm)
Compatible
Quencher
6-FAM™ 494 515 BHQ-1, DABCYL
Fluorescein 495 520 BHQ-1, DABCYL
JOE™ 520 548 BHQ-1, DABCYL
TET™ 521 536 BHQ-1, DABCYL
HEX™ 535 555 BHQ-1, DABCYL
Cy3® 550 570 BHQ-2, DABCYL
ROX™ 573 602 BHQ-2, DABCYL
Texas Red® 583 603 BHQ-2, DABCYL
Cy5® 651 674 BHQ-3, DABCYL
Cy5.5® 675 694 BHQ-3, DABCYL