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Sigma-Proligo
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Oligonucleotide Synthesis > Dual-Labeled Probes

제품명 Dual-Labeled Probes
브랜드 Sigma Proligo
보관 -20 ° C
Cat.No.   Product Description Size 장바구니/견적요청/기타문의
Dual-Labeled Probes      
Locked Nucleic Acid® (LNA) Fluorescent Probes      

 

Choose Dual-Labeled Probes for:

  • Microarray validation
  • Multiply target genes / few samples
  • Pathogen detection
  • Multiplexing
  • Viral load quantification
  • Gene expression analysis
  • Gene copy determination

Benefits of Using Dual-Labeled Probes include:

  • Design simplicity for sequence specificity
  • Increased sensitivity
  • Extensive availability of fluorophore combinations

Add LNA to Your Probe for:

  • Increased thermal stability and hybridization specificity
  • Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
  • Easier and more sensitive probe designs for problematic target sequences

How Dual-Labeled Probes Work

A Dual-Labeled Probe is a single-stranded oligonucleotide labeled with two different dyes. A reporter dye is located at the 5’ end and a quencher molecule located at the 3' end. The quencher molecule inhibits the natural fluorescence emission of the reporter dye by Forster-type energy transfer. The illustration below depicts the mechanism.

The primer is elongated by the polymerase and the probe binds to the specific DNA template, hydrolysis releases the reporter dye from the probe/target hybrid, causing an increase of fluorescence. The measured fluorescence signal is directly proportional to the amount of target DNA.


Product Features include:

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Custom formats available (normalization, special plates, etc.)

Guaranteed Yields
Guaranteed
OD Yield
Approx. No.
of nmoles
Approx. No.
of µg
Approx. No.
of Reactions
*
1 4 32 800
3 12 96 2,400
5 20 160 4,000
10 40 320 8,000
*Estimate is based on 4 nmoles or 32 µg for 1 OD and 200 nM in 25 µl reaction (5.0 pmol/reaction). Estimate is based on an average sequence length of 25 bases.

Spectral Properties Table
Dye Max.
EX (nm)
Max.
EM (nm)
Compatible
Quencher
6-FAM™ 494 515 BHQ-1, TAMRA
JOE™ 520 548 BHQ-1, TAMRA
TET 521 536 BHQ-1, TAMRA
Cal Fluor® Gold 5401 522 541 BHQ-1
HEX2 535 555 BHQ-1, TAMRA
Cal Fluor® Orange 5602 540 561 BHQ-1
TAMRA™ 555 576 BHQ-2
Cy3® 550 570 BHQ-2
Quasar® 5703 548 566 BHQ-2
Cal Fluor® Red 5904 565 588 BHQ-2
ROX™ 573 602 BHQ-2
Texas Red® 583 603 BHQ-2
Cy5® 651 674 BHQ-3
Quasar 6705 647 667 BHQ-3
Cy5.5® 675 694 BHQ-3
1JOE/TET alternative
2VIC® alternative